Mutational profiling (MaP) has become perhaps the most accurate and certainly most direct way of reading out chemical modifications in nucleic acids. ShapeMapper 2 is a completely rewritten version of our software for reading out the results of an RNA chemical probing experiment by massively parallel sequencing. ShapeMapper 2 integrates careful handling of all classes of adduct-induced sequence changes, sequence variant correction, basecall quality filters, and quality-control warnings to now identify RNA adduct sites as accurately as achieved by careful manual analysis of electrophoresis data, the prior highest-accuracy standard. ShapeMapper 2 can be used to read out SHAPE, DMS, RING, and many other classes of experiments.
module spider shapemapper to find out what environment modules are available for this application.
- HPC_SHAPEMAPPER_DIR - installation directory
- HPC_SHAPEMAPPER_BIN - executable directory
- HPC_SHAPEMAPPER_DOC - documentation directory
- HPC_SHAPEMAPPER_EXE - test data directory
If you publish research that uses shapemapper you have to cite it as follows: