PEAR assembles Illumina paired-end reads if the DNA fragment sizes are smaller than twice the length of reads. PEAR can assemble 95% of reads with 35-bp mean overlap with a false-positive rate of 0.004. PEAR also works with multiplexed data sets where the true underlying DNA fragment size varies. PEAR has an extremely low false-positive rate of 0.0003 on data sets where no overlap exists between the two reads (i.e. when DNA fragment sizes are larger than twice the read length).
- Validated 4/5/2018