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nucdiff website  

NucDiff locates and categorizes differences between two closely related nucleotide sequences. It is able to deal with very fragmented genomes, structural rearrangements and various local differences. These features make NucDiff to be perfectly suitable to compare assemblies with each other or with available reference genomes.

Environment Modules

Run module spider nucdiff to find out what environment modules are available for this application.

System Variables

  • HPC_NUCDIFF_DIR - installation directory
  • HPC_NUCDIFF_BIN - executable directory
  • HPC_NUCDIFF_DOC - documentation directory


If you publish research that uses nucdiff you have to cite it as follows:

Kurtz S et al. Versatile and open software for comparing large genomes. Genome Biol. 2004;5(2):R12. doi 10.1186/gb-2004-5-2-r12.