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subread website  

The Subread package: a tool kit for processing next-gen sequencing data

Required Modules


  • subread

System Variables

  • HPC_SUBREAD_DIR - installation directory


If you publish research that uses subread you have to cite it as follows:

If you use the Subread or Subjunc aligners, please cite: Liao Y, Smyth GK and Shi W (2013). The Subread aligner: fast, accurate and scalable read mapping by seed-and-vote. Nucleic Acids Research, 41(10):e108

If you use the featureCounts program, please cite: Liao Y, Smyth GK and Shi W (2014). featureCounts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinformatics, 30(7):923-30