RSeQC

Description

rseqc website  

RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput sequence data especially RNA-seq data. “Basic modules” quickly inspect sequence quality, nucleotide composition bias, PCR bias and GC bias, while “RNA-seq specific modules” investigate sequencing saturation status of both splicing junction detection and expression estimation, mapped reads clipping profile, mapped reads distribution, coverage uniformity over gene body, reproducibility, strand specificity and splice junction annotation

Required Modules

Serial

• rseqc

System Variables

• HPC_RSEQC_DIR - installation directory

Citation

If you publish research that uses rseqc you have to cite it as follows:

Wang L, Wang S, Li W* RSeQC: quality control of RNA-seq experiments Bioinformatics (2012) 28 (16): 2184-2185. doi: 10.1093/bioinformatics/bts356 pubmed