Difference between revisions of "PASA"

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{{App_Module|app={{#var:app}}|intel={{#var:intel}}|mpi={{#var:mpi}}}}|}}
 
{{App_Module|app={{#var:app}}|intel={{#var:intel}}|mpi={{#var:mpi}}}}|}}
 
* HPC_PASA_BIN - executable directory.
 
* HPC_PASA_BIN - executable directory.
 +
* HPC_PASA_CONF - config file directory.
 
{{#if: {{#var: exe}}|==How To Run==
 
{{#if: {{#var: exe}}|==How To Run==
 
===Tools and variables===
 
===Tools and variables===
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* The MySQL server used by PASA has been configured to allow the pasaadmin user to create databases with names that end in _pasa only. So, to run pasa your configuration file such as alignAssembly.config or conf.txt must have MySQL configuration similar to the following entry:
 
* The MySQL server used by PASA has been configured to allow the pasaadmin user to create databases with names that end in _pasa only. So, to run pasa your configuration file such as alignAssembly.config or conf.txt must have MySQL configuration similar to the following entry:
 
  MYSQLDB=test_run_pasa
 
  MYSQLDB=test_run_pasa
 +
 +
The example configuration files are located in <code>/apps/pasa/20110520/pasa_conf</code> ($HPC_PASA_CONF) directory.
 
|}}
 
|}}
 
{{#if: {{#var: conf}}|==Configuration==
 
{{#if: {{#var: conf}}|==Configuration==

Revision as of 17:04, 17 February 2012

Description

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The Program to Assemble Spliced Alignments (PASA) is used to automatically incorporate ESTs and full-length cDNAs into gene structure annotations, in the process annotating UTRs, alternative splicing variations, and polyadenylation sites. Template:App Location

Available Versions

  • 20110520

Running the application using modules

To use pasa with the environment modules system at HPC the following commands are available:

Get module information for pasa:

$module spider pasa

Load the default application module:

$module load pasa

The modulefile for this software adds the directory with executable files to the shell execution PATH and sets the following environment variables:

  • HPC_PASA_DIR - directory where pasa is located.
  • HPC_PASA_BIN - executable directory.
  • HPC_PASA_CONF - config file directory.

How To Run

Tools and variables

  • UNIVECDB environment variable is set to the location of the UniVec database for use by seqclean.

Databases

  • The MySQL server used by PASA has been configured to allow the pasaadmin user to create databases with names that end in _pasa only. So, to run pasa your configuration file such as alignAssembly.config or conf.txt must have MySQL configuration similar to the following entry:
MYSQLDB=test_run_pasa

The example configuration files are located in /apps/pasa/20110520/pasa_conf ($HPC_PASA_CONF) directory.