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Lima, the PacBio barcode demultiplexer, is the standard tool to identify barcode sequences in PacBio single-molecule sequencing data. Starting in SMRT Link v5.1.0, it is the tool that powers the Demultiplex Barcodes GUI-based analysis application. Previous versions of SMRT Link called lima's predecessors, pbbarcode and bam2bam, for demultiplexing. This new tool provides a better end-to-end user experience for analysis of multiplexed samples.

Environment Modules

Run module spider lima to find out what environment modules are available for this application.

System Variables

  • HPC_LIMA_DIR - installation directory
  • HPC_LIMA_BIN - executable directory