Difference between revisions of "KisSplice"
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Latest revision as of 19:33, 15 August 2022
Description
KisSplice is a software that enables to analyse RNA-seq data with or without a reference genome. It is an exact local transcriptome assembler that allows to identify SNPs, indels and alternative splicing events. It can deal with an arbitrary number of biological conditions, and will quantify each variant in each condition. It has been tested on Illumina datasets of up to 1G reads. Its memory consumption is around 5Gb for 100M reads. KisSplice is not a full-length transcriptome assembler. This means that it will output the variable regions of the transcripts, not reconstruct them entirely.
Environment Modules
Run module spider kissplice to find out what environment modules are available for this application.
System Variables
- HPC_KISSPLICE_DIR - installation directory
Citation
If you publish research that uses kissplice you have to cite it as follows: