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Bracken (Bayesian Reestimation of Abundance with KrakEN) is a highly accurate statistical method that computes the abundance of species in DNA sequences from a metagenomics sample. Braken uses the taxonomy labels assigned by Kraken, a highly accurate metagenomics classification algorithm, to estimate the number of reads originating from each species present in a sample. Kraken classifies reads to the best matching location in the taxonomic tree, but does not estimate abundances of species. We use the Kraken database itself to derive probabilities that describe how much sequence from each genome is identical to other genomes in the database, and combine this information with the assignments for a particular sample to estimate abundance at the species level, the genus level, or above. Combined with the Kraken classifier, Bracken produces accurate species- and genus-level abundance estimates even when a sample contains two or more near-identical species.

Environment Modules

Run module spider bracken to find out what environment modules are available for this application.

System Variables

  • HPC_BRACKEN_DIR - installation directory
  • HPC_BRACKEN_BIN - executable directory
  • HPC_BRACKEN_DOC - documentation directory
  • HPC_BRACKEN_EXE - sample data directory


If you publish research that uses bracken you have to cite it as follows: