MarginPolish
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Description
MarginPolish is a graph-based assembly polisher. It iteratively finds multiple probable alignment paths for run-length-encoded reads and uses these to generate a refined sequence. It takes as input a FASTA assembly and an indexed BAM (ONT reads aligned to the assembly), and it produces a polished FASTA assembly.
While MarginPolish serves as a standalone assembly polisher, it is also part of an assembly pipeline which includes an ultrafast nanopore assembler Shasta and a multi-task RNN polisher HELEN. HELEN operates on images generated by MarginPolish.
Environment Modules
Run module spider marginpolish to find out what environment modules are available for this application.
System Variables
- HPC_{{#uppercase:marginpolish}}_DIR - installation directory
- HPC_{{#uppercase:marginpolish}}_BIN - executable directory