SEQuel
Revision as of 21:05, 4 February 2016 by Moskalenko (talk | contribs)
Description
SEQuel is a tool for correcting errors (i.e., insertions, deletions, and substitutions) in contigs output from assembly. While assemblies of next generation sequencing (NGS) data are accurate, they still contain a substantial number of errors that need to be corrected after the assembly process. The algorithm behind SEQuel makes use of a graph structure called the positional de Bruijn graph, which models k-mers within reads while incorporating their approximate positions into the model.
Required Modules
Serial
- sequel
System Variables
- HPC_{{#uppercase:sequel}}_DIR - installation directory
How To Run
Use the 'sequel' script we provide instead of the full 'java -jar...' command.
If needed increase the available memory by setting 'Xmx' in the Java environment variable in your job script or in the shell. E.g.
export _JAVA_OPTIONS="-Xmx6g ${_JAVA_OPTIONS}"