Reptile

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Description

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Reptile is a software developed in C++ for correcting sequencing errors in short reads from next-gen sequencing platforms. Reptile has several favorable properties:

  • Memory efficiency. Reptile can process input data with sizes larger than main memory. For instance, to process a 160x coverage (3.8GB) Illumina data for E. coli it requires only ~1GB memory, which is easily available in a desktop computer.
  • High speed. Processing Illumina data for a microbe typically takes 0.5hr ~ 2hrs, depending on the number and the quality of reads.
  • Can handle reads containing non-acgt characters and reads with non-equal length.
  • Makes simple use of quality score information.
  • Reptile has been developed by Xiao Yang, Karin Dorman and Srinivas Aluru.


Upstream documentation for reptile. Template:App Location

Running the application using modules

To use reptile with the environment modules system at HPC the following commands are available:

Get module information for reptile:

$module spider reptile

Load the default application module:

$module load reptile

The modulefile for this software adds the directory with executable files to the shell execution PATH and sets the following environment variables:

  • HPC_REPTILE_DIR - directory where reptile is located.