Difference between revisions of "Tophat"
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<!--HPC pre-built Bowtie Indexes are located in ''/scratch/hpc/bio/bowtie''. They should be available for bowtie use automatically. If you have additional indexes that could be of use to UF Biological Community please contact us and we'll add them to the reference file space.--> | <!--HPC pre-built Bowtie Indexes are located in ''/scratch/hpc/bio/bowtie''. They should be available for bowtie use automatically. If you have additional indexes that could be of use to UF Biological Community please contact us and we'll add them to the reference file space.--> | ||
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==Available Versions== | ==Available Versions== | ||
* 1.3.0. | * 1.3.0. | ||
Revision as of 02:00, 10 August 2012
Description
TopHat is a fast splice junction mapper for RNA-Seq reads. It aligns RNA-Seq reads to mammalian-sized genomes using the ultra high-throughput short read aligner Bowtie, and then analyzes the mapping results to identify splice junctions between exons.
Tophat is a part of the Tuxedo suite.
Available Versions
- 1.3.0.
- 1.3.3 beta.
- 1.4.1 (default).
Running the application using modules
To use tophat with the environment modules system at HPC the following commands are available:
Get module information for tophat:
$module spider tophat
Load the default application module:
$module load tophat
The modulefile for this software adds the directory with executable files to the shell execution PATH and sets the following environment variables:
- HPC_TOPHAT_DIR - directory where tophat is located.
- HPC_TOPHAT_BIN - executable directory
- HPC_TOPHAT_DOC - documents directory