RELION

Description

relion website  

RELION (for REgularised LIkelihood OptimisatioN, pronounce rely-on) is a stand-alone computer program that employs an empirical Bayesian approach to refinement of (multiple) 3D reconstructions or 2D class averages in electron cryo-microscopy (cryo-EM). It is developed in the group of Sjors Scheres at the MRC Laboratory of Molecular Biology. Briefly, the ill-posed problem of 3D-reconstruction is regularised by incorporating prior knowledge: the fact that macromolecular structures are smooth, i.e. they have limited power in the Fourier domain. In the corresponding Bayesian framework, many parameters of a statistical model are learned from the data, which leads to objective and high-quality results without the need for user expertise. The underlying theory is given in Scheres (2012) JMB.

Environment Modules

Run module spider relion to find out what environment modules are available for this application.

System Variables

• HPC_RELION_DIR - installation directory

Additional Information

Note

Relion will not open its starting window in a GUI Session if there is no project file in the current working directory. While we verified that both 'launch_gui_session -e relion' and 'launch_relion_session' should overcome this issue if you run into this problem a simple fix to get the initial startup is to copy a sample project file from /apps/relion/conf/default_pipeline.star to the current working directory before launching a Relion session.

Citation

If you publish research that uses relion you have to cite it as follows:

RELION: Implementation of a Bayesian approach to cryo-EM structure determination