Difference between revisions of "Tophat"
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Moskalenko (talk | contribs) m (Text replacement - "#uppercase" to "uc") |
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*{{#var:app}} | *{{#var:app}} | ||
==System Variables== | ==System Variables== | ||
| − | * HPC_{{ | + | * HPC_{{uc:{{#var:app}}}}_DIR - installation directory |
* HPC_TOPHAT_BIN - executable directory | * HPC_TOPHAT_BIN - executable directory | ||
* HPC_TOPHAT_DOC - documents directory | * HPC_TOPHAT_DOC - documents directory | ||
Revision as of 21:29, 6 December 2019
Description
TopHat is a fast splice junction mapper for RNA-Seq reads. It aligns RNA-Seq reads to mammalian-sized genomes using the ultra high-throughput short read aligner Bowtie, and then analyzes the mapping results to identify splice junctions between exons.
Tophat is a part of the Tuxedo suite.
Required Modules
Serial
- tophat
System Variables
- HPC_TOPHAT_DIR - installation directory
- HPC_TOPHAT_BIN - executable directory
- HPC_TOPHAT_DOC - documents directory
How To Run
Note: If your data is compressed your job script should add another ppn to accommodate the external gzip process tophat will run. To be safe, add another ppn in any case.
Validation
- Validated 4/5/2018