Difference between revisions of "RELION"
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Revision as of 18:43, 29 November 2017
Description
RELION (for REgularised LIkelihood OptimisatioN, pronounce rely-on) is a stand-alone computer program that employs an empirical Bayesian approach to refinement of (multiple) 3D reconstructions or 2D class averages in electron cryo-microscopy (cryo-EM). It is developed in the group of Sjors Scheres at the MRC Laboratory of Molecular Biology. Briefly, the ill-posed problem of 3D-reconstruction is regularised by incorporating prior knowledge: the fact that macromolecular structures are smooth, i.e. they have limited power in the Fourier domain. In the corresponding Bayesian framework, many parameters of a statistical model are learned from the data, which leads to objective and high-quality results without the need for user expertise. The underlying theory is given in Scheres (2012) JMB.
Required Modules
Parallel (MPI)
- gcc/5.2.0
- openmpi/1.10.2
- relion
System Variables
- HPC_{{#uppercase:relion}}_DIR - installation directory
Additional Information
- Note
Relion will not open its starting window in a GUI Session if there is no project file in the current working directory. A simple fix to get the initial startup is to copy a sample project file from /apps/relion/conf/default_pipeline.star to the current working directory before launching a relion session.
Citation
If you publish research that uses relion you have to cite it as follows:
RELION: Implementation of a Bayesian approach to cryo-EM structure determination