Difference between revisions of "RELION"
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Revision as of 16:50, 28 June 2016
Description
RELION (for REgularised LIkelihood OptimisatioN, pronounce rely-on) is a stand-alone computer program that employs an empirical Bayesian approach to refinement of (multiple) 3D reconstructions or 2D class averages in electron cryo-microscopy (cryo-EM). It is developed in the group of Sjors Scheres at the MRC Laboratory of Molecular Biology. Briefly, the ill-posed problem of 3D-reconstruction is regularised by incorporating prior knowledge: the fact that macromolecular structures are smooth, i.e. they have limited power in the Fourier domain. In the corresponding Bayesian framework, many parameters of a statistical model are learned from the data, which leads to objective and high-quality results without the need for user expertise. The underlying theory is given in Scheres (2012) JMB.
Required Modules
Parallel (MPI)
- gcc/5.2.0
- openmpi/1.10.2
- relion
System Variables
- HPC_{{#uppercase:relion}}_DIR - installation directory
Citation
If you publish research that uses relion you have to cite it as follows:
RELION: Implementation of a Bayesian approach to cryo-EM structure determination