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tgicl website  

TGI Clustering tools (TGICL): a software system for fast clustering of large EST datasets

This package automates clustering and assembly of a large EST/mRNA dataset. The clustering is performed by a slightly modified version of NCBI's megablast , and the resulting clusters are then assembled using CAP3 assembly program. TGICL starts with a large multi-FASTA file (and an optional peer quality values file) and outputs the assembly files as produced by CAP3

Required Modules

modules documentation


  • tgicl
  • HPC_TGICL_BIN - executable directory
  • HPC_TGICL_CONF - conf directory


By default loading the module for tgicl also loads the latest Perl and CAP3 modules. The main version of cap3 is used for analyses. If you wish to use the cap3 version distributed with tgicl, it is located at $HPC_TGICL_BIN/cap3.tgicl

System Variables

  • HPC_{{#uppercase:tgicl}}_DIR - installation directory

How To Run

  • To copy the default skeleton configuration file to the working directory:
cp $HPC_TGICL_CONF/tgicl.cfg .
  • To get a full list of tgicl commands:
perldoc tgicl
  • Sample run
tgicl -F sample.fasta  -q sample.qual -c 4