SOAPsplice is a tool for genome-wide ab initio detection of splice junction sites from RNA-Seq. The tool performs better than the previous tools by detecting similar numbers of true junctions with lower false positives as the best performing tools in all different situations with different read lengths and coverage. In particular, SOAPsplice performs better by predicting more true junctions with low false positive rate when the coverage is low, which is useful for detecting the junctions for those mRNAs with relatively lower expression level.
module spider soapsplice to find out what environment modules are available for this application.
- HPC_SOAPSPLICE_DIR - installation directory