View source for RELION

[[Category:Software]][[Category:Biology]][[Category:Imaging]]
{|<!--CONFIGURATION: REQUIRED-->
|{{#vardefine:app|relion}}
|{{#vardefine:url|http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page}}
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{{App_Description|app={{#var:app}}|url={{#var:url}}|name={{#var:app}}}}|}}

RELION (for REgularised LIkelihood OptimisatioN, pronounce rely-on) is a stand-alone computer program that employs an empirical Bayesian approach to refinement of (multiple) 3D reconstructions or 2D class averages in electron cryo-microscopy (cryo-EM). It is developed in the group of Sjors Scheres at the MRC Laboratory of Molecular Biology. Briefly, the ill-posed problem of 3D-reconstruction is regularised by incorporating prior knowledge: the fact that macromolecular structures are smooth, i.e. they have limited power in the Fourier domain. In the corresponding Bayesian framework, many parameters of a statistical model are learned from the data, which leads to objective and high-quality results without the need for user expertise. The underlying theory is given in Scheres (2012) JMB.

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==Required Modules==
===Parallel (MPI)===
* gcc/5.2.0
* openmpi/1.10.2
* {{#var:app}}
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==System Variables==
* HPC_{{uc:{{#var:app}}}}_DIR - installation directory
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{{#if: {{#var: conf}}|==Configuration==
See the [[{{PAGENAME}}_Configuration]] page for {{#var: app}} configuration details.
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{{#if: {{#var: exe}}|==Additional Information==

;Note:
Relion will not open its starting window in a [[GUI Programs|GUI Session]] if there is no project file in the current working directory. While we verified that both '<code>launch_gui_session -e relion</code>' and '<code>launch_relion_session</code>' should overcome this issue if you run into this problem a simple fix to get the initial startup is to copy a sample project file from /apps/relion/conf/default_pipeline.star to the current working directory before launching a Relion session.

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{{#if: {{#var: pbs}}|==PBS Script Examples==
See the [[{{PAGENAME}}_PBS]] page for {{#var: app}} PBS script examples.
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<!--Policy-->
{{#if: {{#var: policy}}|==Usage Policy==

WRITE USAGE POLICY HERE (Licensing, usage, access).

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<!--Performance-->
{{#if: {{#var: testing}}|==Performance==

WRITE_PERFORMANCE_TESTING_RESULTS_HERE

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<!--Faq-->
{{#if: {{#var: faq}}|==FAQ==
*'''Q:''' **'''A:'''|}}
<!--Citation-->
{{#if: {{#var: citation}}|==Citation==
If you publish research that uses {{#var:app}} you have to cite it as follows:

[http://www.sciencedirect.com/science/article/pii/S1047847712002481 RELION: Implementation of a Bayesian approach to cryo-EM structure determination]

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{{#if: {{#var: installation}}|==Installation==
See the [[{{PAGENAME}}_Install]] page for {{#var: app}} installation notes.|}}
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