ngsCAT (Next Generation Sequencing data Capture Assessment Tool) is a command-line application written in Python which facilitates a comprehensive evaluation of the performance of the capture step in targeted high-throughput sequencing experiments in terms of:
- Sensitivity, which assesses the quality of the coverage on target regions. It is also important to provide a means of estimating how this coverage
would improve by increasing sequencing depth.
- Specificity, which measures how much of the sequencing effort is wasted on
sequencing off-target bases.
- Uniformity, which assesses sequencing biases due to specific genomic
locations or nucleotide composition.
- HPC_NGSCAT_DIR - installation directory
If you publish research that uses ngscat you have to cite it as follows:
Francisco J.López-Domingo, Javier P.Florido, Antonio Rueda, Joaquín Dopazo and Javier Santoyo-López (2014) ngsCAT: a tool to assess the efficiency of targeted enrichment sequencing, Bioinformatics, vol.30, no.12, pp.1767-1768, 2014; doi:10.1093/bioinformatics/btu108.
- Validated 4/5/2018