Difference between revisions of "Mosaik"

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Revision as of 16:32, 10 August 2012

Description

mosaik website  

MOSAIK is a reference-guided assembler comprising of four main modular programs:

  • MosaikBuild
  • MosaikAligner
  • MosaikSort
  • MosaikText

MosaikBuild converts various sequence formats into Mosaik’s native read format. MosaikAligner pairwise aligns each read to a specified series of reference sequences. MosaikSort resolves paired-end reads and sorts the alignments by the reference sequence coordinates. Finally, MosaikText converts alignments to different text-based formats.

At this time, the workflow consists of supplying sequences in FASTA, FASTQ, Illumina Bustard & Gerald, or SRF file formats and producing results in the BLAT axt, the BAM/SAM, the UCSC Genome Browser bed, or the Illumina ELAND formats.

Execution Environment and Modules

To use mosaik with the environment modules system at HPC the following commands are available:

Get module information for mosaik:

$module spider mosaik

Load the default application module:

$module load mosaik

The modulefile for this software adds the directory with executable files to the shell execution PATH and sets the following environment variables:

  • HPC_MOSAIK_DIR - directory where mosaik is located.
  • HPC_MOSAIK_BIN - executable file directory