Difference between revisions of "Mosaik"

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*{{#var:app}}
 
*{{#var:app}}
 
==System Variables==
 
==System Variables==
* HPC_{{#uppercase:{{#var:app}}}}_DIR - installation directory
+
* HPC_{{#uppercase:{{#var:app}}}}_DIR - installation directory.
* HPC_MOSAIK_BIN - executable file directory
+
* HPC_MOSAIK_BIN - executable file directory.
* HPC_MOSAIK_NET - network file directory
+
* HPC_MOSAIK_NET - network file directory.
 +
* MOSAIK_TMP - default temporary file directory. Note that HiPerGator2 nodes are diskless, so MOSAIK_TMP must not be set to /tmp.
 
{{#if: {{#var: exe}}|==How To Run==
 
{{#if: {{#var: exe}}|==How To Run==
 
If you would like to use the provided network files with MosaikAligner use the $HPC_MOSAIK_NET variable for the location of network file directory. E.g.
 
If you would like to use the provided network files with MosaikAligner use the $HPC_MOSAIK_NET variable for the location of network file directory. E.g.

Revision as of 17:29, 9 September 2016

Description

mosaik website  

MOSAIK is a reference-guided assembler comprising of four main modular programs:

  • MosaikBuild
  • MosaikAligner
  • MosaikSort
  • MosaikText

MosaikBuild converts various sequence formats into Mosaik’s native read format. MosaikAligner pairwise aligns each read to a specified series of reference sequences. MosaikSort resolves paired-end reads and sorts the alignments by the reference sequence coordinates. Finally, MosaikText converts alignments to different text-based formats.

At this time, the workflow consists of supplying sequences in FASTA, FASTQ, Illumina Bustard & Gerald, or SRF file formats and producing results in the BLAT axt, the BAM/SAM, the UCSC Genome Browser bed, or the Illumina ELAND formats.

Required Modules

modules documentation

Serial

  • mosaik

System Variables

  • HPC_{{#uppercase:mosaik}}_DIR - installation directory.
  • HPC_MOSAIK_BIN - executable file directory.
  • HPC_MOSAIK_NET - network file directory.
  • MOSAIK_TMP - default temporary file directory. Note that HiPerGator2 nodes are diskless, so MOSAIK_TMP must not be set to /tmp.

How To Run

If you would like to use the provided network files with MosaikAligner use the $HPC_MOSAIK_NET variable for the location of network file directory. E.g.

MosaikAligner -annpe $HPC_MOSAIK_NET/2.1.26.pe.100.0065.ann \
-annse $HPC_MOSAIK_NET/2.1.26.se.100.005.ann -in ...