Difference between revisions of "Bcl2fastq"
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Revision as of 20:45, 10 June 2022
Description
bcl2fastq conversion software can be used to both demultiplex data and convert BCL files to FASTQ files. This software requires a Linux operating system. The bcl2fastq2 software can be used to convert BCL files from all Illumina sequencing systems running RTA version 2.0 and above. This is a change from the original information, which indicated that v2.16 could be used with all sequencers running RTA 1.18 and above: that information is no longer correct. For conversion of data generated on Illumina sequencing systems using versions of RTA before RTA 2.0, use bcl2fastq v1.8.4.
Environment Modules
Run module spider bcl2fastq to find out what environment modules are available for this application.
System Variables
- HPC_BCL2FASTQ_DIR - installation directory