Trinity
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Trinity is an application for de novo reconstruction of transcriptomes from RNA-Seq data. Its home page is at http://trinityrnaseq.sourceforge.net/.
Installation
Trinity contains three applications - Inchworm, Chrysalis, and Butterfly. They are meant to be install in-place. To install, just download Trinity from their website, untar it, and type 'make'.
Testing
There are two tests which come with Trinity:
/apps/trinity/r20110519/sample_data/runMe.sh /apps/trinity/r20110519/Butterfly/src/runExample.sh
The former tests the whole flow, and the latter tests Butterfly only.