View source for SRA

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[[Category:Software]][[Category:Biology]][[Category:NGS]]
{|<!--Main settings - REQUIRED-->
|{{#vardefine:app|sra}}
|{{#vardefine:url|http://trace.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?cmd=show&f=software&m=software&s=software}}
|{{#vardefine:exe|1}} <!--Present manual instructions for running the software -->
|{{#vardefine:conf|}} <!--Enable config wiki page link - {{#vardefine:conf|1}} = ON/conf|}} = OFF-->
|{{#vardefine:pbs|}} <!--Enable PBS script wiki page link-->
|{{#vardefine:policy|}} <!--Enable policy section -->
|{{#vardefine:testing|}} <!--Enable performance testing/profiling section -->
|{{#vardefine:faq|}} <!--Enable FAQ section -->
|{{#vardefine:citation|}} <!--Enable Reference/Citation section -->
|}
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<!--Description-->
{{#if: {{#var: url}}|
{{App_Description|app={{#var:app}}|url={{#var:url}}|name={{#var:app}}}}|}}

This is the NCBI Short Read Archive Toolkit.

;Note: sra will create a $HOME/ncbi/public directory and cache the prefetched data files there. However, home directory has a 40gb limit and its use for job data storage is a violation of the [https://www.rc.ufl.edu/about/policies/storage/ UFRC storage policy]. You must change that location to a directory in your ufrc space before running the sra toolkit. The official approach is to use the vdb-config tool
 vdb-config -i
and change the directory to, for example, /blue/$GROUP/$USER/ncbi/public. See the [https://github.com/ncbi/sra-tools/wiki/Toolkit-Configuration SRA Toolkit Configuration Documentation] for more details.

Alternatively, create an 'ncbi' directory in your /blue space and symlink it to ~/ncbi. E.g.

 $ mkdir /blue/mygroup/$USER/ncbi
 $ ln -s /blue/mygroup/$USER/ncbi ~/ncbi

==Uploads==
It appears that data uploads to NCBI only work from login servers. Start a screen session before beginning an upload if there are any concerns about being disconnected.
<!--Modules-->
==Environment Modules==
Run <code>module spider {{#var:app}}</code> to find out what environment modules are available for this application.
==System Variables==
* HPC_{{uc:{{#var:app}}}}_DIR - installation directory
* HPC_SRA_BIN - location of the executables directory
* HPC_SRA_DOC - location of the documentation directory
<!--Additional-->
{{#if: {{#var: exe}}|==Additional Information==
===Aspera Connect===
To download SRA data you can use the "ascp" utility from the [http://asperasoft.com/downloads/ Aspera Connect] browser plugin package. We have a copy installed and provided by the sra module. A wrapper script ''ascp.sh'' that automatically uses the ssh key is available. For instance:
 ascp.sh -QT anonftp@ftp-private.ncbi.nlm.nih.gov:/genomes/Bacteria/all.faa.tar.gz faa
will download the all.faa.tar.gz archive to the faa directory. 

'''Note:''' if the download fails to run with a "Session Stop (Error: Client unable to connect to server (check UDP port and firewall))"
error ''make sure you are running aspera connect on a login node''. If you are already doing the transfer on a login node please submit a support request. This means that the remote site has not been allowed through the firewall. Please be sure to include the path to a script you used to run the data transfer command into the request. Do not put any sensitive information like passwords, keys, and such into the request.
|}}
{{#if: {{#var: conf}}|==Configuration==
See the [[{{PAGENAME}}_Configuration]] page for {{#var: app}} configuration details.|}}
{{#if: {{#var: pbs}}|==PBS Script Examples==
See the [[{{PAGENAME}}_PBS]] page for {{#var: app}} PBS script examples.|}}
{{#if: {{#var: policy}}|==Usage Policy==
WRITE USAGE POLICY HERE (perhaps templates for a couple of main licensing schemes can be used)|}}
{{#if: {{#var: testing}}|==Performance==
WRITE PERFORMANCE TESTING RESULTS HERE|}}
{{#if: {{#var: faq}}|==FAQ==
*'''Q:''' **'''A:'''|}}
{{#if: {{#var: citation}}|==Citation==
If you publish research that uses {{{app}}} you have to cite it as follows:
WRITE CITATION HERE
|}}